A 3D Printer That Generates Human Embryonic Stem Cells
2013-02-07 0:00

By Rebecca Boyle | io9.com


3-D printers can produce gun parts, aircraft wings, food and a lot more, but this new 3-D printed product may be the craziest thing yet: human embryonic stem cells. Using stem cells as the "ink" in a 3-D printer, researchers in Scotland hope to eventually build 3-D printed organs and tissues. A team at Heriot-Watt University used a specially designed valve-based technique to deposit whole, live cells onto a surface in a specific pattern.

This article originally appeared at Popular Science.

The cells were floating in a "bio-ink," to use the terminology of the researchers who developed this technique. They were able to squeeze out tiny droplets, containing five cells or fewer per droplet, in a variety of shapes and sizes. To produce clumps of cells, the team printed out cells first and then overlaid those with cell-free bio-ink, resulting in larger droplets or spheroids of cells. The cells would group together inside these spheroids. Spheroid size is key, because stem cells need certain conditions to work properly. This is why very precisely controlled 3-D printing could be so valuable for stem cell research.

After being squeezed out of a thin valve, the cells were still alive and viable, and able to transform into any other cell in the body, the researchers say. It’s the first time anyone has printed human embyronic stem cells, said lead researcher Will Wenmiao Shu, a professor at Heriot-Watt. But ... why?

Eventually, they could be used to print out new tissues, or as filler inside existing organs, which would be regenerated. It could even serve to limit animal testing for new drug compounds, allowing them to be tested on actual human tissue, said Jason King, business development manager at Roslin Cellab, one of the research partners. "In the longer term, [it could] provide organs for transplant on demand, without the need for donation and without the problems of immune suppression and potential organ rejection," he said in a statement.

The team took stem cells from an embryonic kidney and from a well-studied embryonic cell line, and grew them in culture. They had to build a custom reservoir — let’s call it an inkwell — to safely house the delicate cells, and then they added some large-diameter nozzles. A pressurized air supply pumps the cells from the inkwell into the valves, which contain pressurized nozzles on the end. The team could control the amount of cells dispensed by changing any of the factors, including the pneumatic pressure, nozzle diameter or length of time the nozzle stayed open.

At first the researchers printed droplets, but ultimately, they were so precise that they made cell spheroids in a variety of shapes and sizes, like the university logo above. One interesting wrinkle: The cells also formed spheroids in the inkwells. More work needs to be done to explain that.

The researchers also took several steps to make sure the cells survived the printing process. Examining the results of several experiments, they found 99 percent of the cells were still viable after running through the valve-based printer.

[...]

Read the full article at: io9.com





In partnership with Roslin Cellab, the university’s biomedical engineering group came up with a valve-based printing technique that produces highly-viable, uniformly-sized droplets of stem cells. Most importantly, though, the cells maintain their pluripotency — the ability to differentiate into every adult human cell type. While the prospect of lab-grown custom organs is incredibly exciting, the group is careful to temper its outlook, noting that any such application would be "in the longer term."
Source: TheVerge.com



Related Articles
Bioengineers ’3D Print’ Living Human Embryonic Stem Cells for First Time
This Child Has A Robot Hand Made With 3D Printers
Long-Gone Mollusk Comes to Life with 3D Printer
3D printer creates physical model of fetus for expecting parents
Artificial blood vessels created on a 3D printer


Latest News from our Front Page

Activists Plan to Burn American Flags in New York City Ahead of Fourth of July
2015-07-01 1:52
Burning the flag in Charleston, South Carolina on June 22, 2015 A group calling for the immediate disarmament of the New York Police Department plans to burn American flags in a Brooklyn park on Wednesday, just days before the Fourth of July holiday. “Disarm NYPD” announced the “Burn the American Flags” event on Facebook, inviting individuals to join the organization at Fort ...
Ruiz: Diversity of New York City languages makes staying involved in kids’ education difficult for immigrant parents
2015-07-01 1:08
Immigrant parent seek better translation and interpretation services from the Department of Education at the New York Immigration Coalition. New Yorkers, who come from every corner of the world, speak 180 languages. Not surprisingly, nearly half of public school students speak a language other than English at home. This makes life difficult for the city's Department of Education, which, according to federal ...
Ethnic Studies to Become Mandatory in Sacramento Schools by 2016
2015-06-30 22:36
The fourth most diverse school district in the nation is finally adding an ethnic studies graduation requirement—in 2020. The Sacramento City Unified School District's Board of Trustees unanimously approved the proposal from its Student Advisory Council on June 4. The proposal will create an ethnic studies pilot program for fall 2016. In collaboration with community organizations, local university professors and college students, ...
It Begins: New Calls To Strip Churches of Tax Exempt Status After Same-Sex Marriage Ruling
2015-06-30 20:22
For years conservatives and proponents of religious liberty in America have warned that if same-sex marriage became legal, the left would then pursue revoking the tax exempt status for religious institutions, particularly Christian churches, around the country. Just days after the Supreme Court ruled in a 5-4 decision that gay marriage is a constitutional right, progressive activists like Mark Oppenheimer of ...
Physician Linking Autism - Vaccines Found Shot Dead.
2015-06-30 3:38
James Jeffrey Bradstreet was a hero to many parents. He spent a great part of his life trying to fight for those children who sustained injuries from vaccines via the creation of medical procedures. This included his very own son. Clearly, his ideas and concepts and science were not accepted by all and created a great many heated debates online. On ...
More News »