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A 3D Printer That Generates Human Embryonic Stem Cells 2013-02-07 0:00
3-D printers can produce gun parts, aircraft wings, food and a lot more, but this new 3-D printed product may be the craziest thing yet: human embryonic stem cells. Using stem cells as the "ink" in a 3-D printer, researchers in Scotland hope to eventually build 3-D printed organs and tissues. A team at Heriot-Watt University used a specially designed valve-based technique to deposit whole, live cells onto a surface in a specific pattern.
This article originally appeared at Popular Science.
The cells were floating in a "bio-ink," to use the terminology of the researchers who developed this technique. They were able to squeeze out tiny droplets, containing five cells or fewer per droplet, in a variety of shapes and sizes. To produce clumps of cells, the team printed out cells first and then overlaid those with cell-free bio-ink, resulting in larger droplets or spheroids of cells. The cells would group together inside these spheroids. Spheroid size is key, because stem cells need certain conditions to work properly. This is why very precisely controlled 3-D printing could be so valuable for stem cell research.
After being squeezed out of a thin valve, the cells were still alive and viable, and able to transform into any other cell in the body, the researchers say. It’s the first time anyone has printed human embyronic stem cells, said lead researcher Will Wenmiao Shu, a professor at Heriot-Watt. But ... why?
Eventually, they could be used to print out new tissues, or as filler inside existing organs, which would be regenerated. It could even serve to limit animal testing for new drug compounds, allowing them to be tested on actual human tissue, said Jason King, business development manager at Roslin Cellab, one of the research partners. "In the longer term, [it could] provide organs for transplant on demand, without the need for donation and without the problems of immune suppression and potential organ rejection," he said in a statement.
The team took stem cells from an embryonic kidney and from a well-studied embryonic cell line, and grew them in culture. They had to build a custom reservoir — let’s call it an inkwell — to safely house the delicate cells, and then they added some large-diameter nozzles. A pressurized air supply pumps the cells from the inkwell into the valves, which contain pressurized nozzles on the end. The team could control the amount of cells dispensed by changing any of the factors, including the pneumatic pressure, nozzle diameter or length of time the nozzle stayed open.
At first the researchers printed droplets, but ultimately, they were so precise that they made cell spheroids in a variety of shapes and sizes, like the university logo above. One interesting wrinkle: The cells also formed spheroids in the inkwells. More work needs to be done to explain that.
The researchers also took several steps to make sure the cells survived the printing process. Examining the results of several experiments, they found 99 percent of the cells were still viable after running through the valve-based printer.
In partnership with Roslin Cellab, the university’s biomedical engineering group came up with a valve-based printing technique that produces highly-viable, uniformly-sized droplets of stem cells. Most importantly, though, the cells maintain their pluripotency — the ability to differentiate into every adult human cell type. While the prospect of lab-grown custom organs is incredibly exciting, the group is careful to temper its outlook, noting that any such application would be "in the longer term."
Russians drop leaflets on ISIS, implying America and Israel is behind them 2015-10-09 4:38
Dire warning: The leaflets (pictured) being dropped by Russian jets warn people to get out of the zone of 'special operations' - and one implies that terror group al-Nusra Front are backed by America and Israel
Syrian army helicopters have dropped tens of thousands of leaflets on ISIS and rebel fighters, warning them to get out now and 'don't make it even ...
College diversity officer charged for #KillAllWhiteMen tweet 2015-10-08 23:16
A U.K. college diversity officer’s utopia would include no white men – after they are all executed.
An employee of Goldsmiths University of London’s independent students’ union was arrested in London Tuesday and charged with sending a menacing or offensive messages via a public network for her “#killallwhitemen” tweets.
Bahar Mustafa, 28, faces one charge each for electronic communications she sent over ...
Paedo deported from UK sneaked back and worked with kids for two years before being caught 2015-10-08 23:50 Landazuri worked with teenagers for two years before he was caught
Juan Guerra Landazuri successfully lived in Britain with his new identity for two years and even got a job helping vulnerable teenagers - despite being banned from working with children for life.
The 44-year-old, originally known as Juan Alejandro Guerra, was jailed for four years in 2002 for two offences of ...
Why Don't More Minorities Hike? 2015-10-08 23:06
As a Bay Area native, I grew up in outdoor spaces. While hiking trails in Tilden Park in the East Bay and in the Marin Headlands in the North Bay, I gained an appreciation of and respect for natural spaces that eventually led me to visit our national parksâ€”Yosemite being my favorite.
It was in Yosemite that I started to notice ...