A 3D Printer That Generates Human Embryonic Stem Cells
2013 02 07

By Rebecca Boyle | io9.com


3-D printers can produce gun parts, aircraft wings, food and a lot more, but this new 3-D printed product may be the craziest thing yet: human embryonic stem cells. Using stem cells as the "ink" in a 3-D printer, researchers in Scotland hope to eventually build 3-D printed organs and tissues. A team at Heriot-Watt University used a specially designed valve-based technique to deposit whole, live cells onto a surface in a specific pattern.

This article originally appeared at Popular Science.

The cells were floating in a "bio-ink," to use the terminology of the researchers who developed this technique. They were able to squeeze out tiny droplets, containing five cells or fewer per droplet, in a variety of shapes and sizes. To produce clumps of cells, the team printed out cells first and then overlaid those with cell-free bio-ink, resulting in larger droplets or spheroids of cells. The cells would group together inside these spheroids. Spheroid size is key, because stem cells need certain conditions to work properly. This is why very precisely controlled 3-D printing could be so valuable for stem cell research.

After being squeezed out of a thin valve, the cells were still alive and viable, and able to transform into any other cell in the body, the researchers say. It’s the first time anyone has printed human embyronic stem cells, said lead researcher Will Wenmiao Shu, a professor at Heriot-Watt. But ... why?

Eventually, they could be used to print out new tissues, or as filler inside existing organs, which would be regenerated. It could even serve to limit animal testing for new drug compounds, allowing them to be tested on actual human tissue, said Jason King, business development manager at Roslin Cellab, one of the research partners. "In the longer term, [it could] provide organs for transplant on demand, without the need for donation and without the problems of immune suppression and potential organ rejection," he said in a statement.

The team took stem cells from an embryonic kidney and from a well-studied embryonic cell line, and grew them in culture. They had to build a custom reservoir — let’s call it an inkwell — to safely house the delicate cells, and then they added some large-diameter nozzles. A pressurized air supply pumps the cells from the inkwell into the valves, which contain pressurized nozzles on the end. The team could control the amount of cells dispensed by changing any of the factors, including the pneumatic pressure, nozzle diameter or length of time the nozzle stayed open.

At first the researchers printed droplets, but ultimately, they were so precise that they made cell spheroids in a variety of shapes and sizes, like the university logo above. One interesting wrinkle: The cells also formed spheroids in the inkwells. More work needs to be done to explain that.

The researchers also took several steps to make sure the cells survived the printing process. Examining the results of several experiments, they found 99 percent of the cells were still viable after running through the valve-based printer.

[...]

Read the full article at: io9.com





In partnership with Roslin Cellab, the university’s biomedical engineering group came up with a valve-based printing technique that produces highly-viable, uniformly-sized droplets of stem cells. Most importantly, though, the cells maintain their pluripotency — the ability to differentiate into every adult human cell type. While the prospect of lab-grown custom organs is incredibly exciting, the group is careful to temper its outlook, noting that any such application would be "in the longer term."
Source: TheVerge.com



Related Articles
Bioengineers ’3D Print’ Living Human Embryonic Stem Cells for First Time
This Child Has A Robot Hand Made With 3D Printers
Long-Gone Mollusk Comes to Life with 3D Printer
3D printer creates physical model of fetus for expecting parents
Artificial blood vessels created on a 3D printer


Latest News from our Front Page

US Silent on Psychologists Role in CIA’s Tortures: Doctors
2014-12-20 21:53
Physicians for Human Rights had not received any response from the US Federal Commission to their call to investigate the role of health professionals in CIA’s torture program, Deputy Director of the organization told Sputnik. December 19 (Sputnik) — US government has not responded to calls to prosecute doctors, who participated in CIA torture program, the Deputy Director of Communications for ...
Ziolebrities: Simon Cowell donates £100,000 to Israeli soldiers to please pregnant jewish girlfriend Lauren Silverman
2014-12-20 21:00
Cowell, 54, is also planning a secret trip to Israel soon as he embraces the Jewish faith of Silverman, 36 Gala: Billionaire Haim Saban with Cowell Simon Cowell has publicly donated nearly £100,000 in support of the Israeli army. The X Factor boss pledged the cash to the Friends of the Israel Defense Forces at a US fund-raiser in Beverly Hills. The lavish gala ...
Former Chief Security Officer for NewsCorp: N. Koreans Not Behind Sony Hack, Interview Leak
2014-12-20 2:17
Hemanshu Nigam, a former federal prosecutor and former chief security officer for NewsCorp/Fox studios, says North Korea isn’t behind the Sony Hack. Nigam gave several bullet points for why the hack was likely an inside job. Attack code borrowed from a previous attack on Seoul, that’s why it’s in Korean. Private hackers typically borrow malicious code from other hackers.Nations state attacks follow ...
Sony Fires Back at Obama: Actually We Did Call the White House – Several Times
2014-12-20 2:13
Sony fired back at Obama after the press conference saying they had several conversations with the Obama White House before and after the movie was canceled. Via The Hollywood Reporter: After President Obama criticized Sony for its decision to cancel The Interview's release after theater chains decided not to show the film, the studio has issued a statement elaborating on the move. “The ...
The Bankster International
2014-12-20 1:55
Geopolitical analysis, the art of explaining power relationships through the prism of impersonal geography, can be a helpful tool for observers of the Great Game – but it also has its limitations. A case in point is the renewed US-Russia confrontation. Think tanks and policy insiders easily sell the narrative that from the dark days of the Cold War to ...
More News »